Cell
Part:BBa_J72213:Design
Designed by: Josh Kittleson Group: Anderson Lab (2012-09-17)
MC1061 O16::repA+FRT-kanR-FRT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1628
Illegal XbaI site found at 2787
Illegal XbaI site found at 3856
Illegal PstI site found at 137
Illegal PstI site found at 2758 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1628
Illegal NheI site found at 1649
Illegal NheI site found at 1672
Illegal PstI site found at 137
Illegal PstI site found at 2758 - 21INCOMPATIBLE WITH RFC[21]Illegal suffix found in sequence at 2743
Illegal EcoRI site found at 1628
Illegal BglII site found at 1637 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1628
Illegal XbaI site found at 2787
Illegal XbaI site found at 3856
Illegal PstI site found at 137
Illegal PstI site found at 2758 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1628
Illegal XbaI site found at 2787
Illegal XbaI site found at 3856
Illegal PstI site found at 137
Illegal PstI site found at 2758 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 34
Design Notes
n/a
Source
n/a